Maipomycin A, a Novel Natural Compound With Promising Anti-biofilm Activity Against Gram-Negative Pathogenic Bacteria

Pathogenic bacterial biofilms play an vital position in recurrent nosocomial and medical device-related infections. As soon as occurred, the advanced construction of the biofilm promotes the event of antibiotic resistance and turns into extraordinarily tough to eradicate. Right here we describe a novel and efficient anti-biofilm compound maipomycin A (MaiA), which was remoted from the metabolites of a uncommon actinomycete pressure Kibdelosporangium phytohabitans XY-R10. Its construction was deduced from analyses of spectral knowledge and confirmed by single-crystal X-ray crystallography.

This pure product demonstrated a broad spectrum of anti-biofilm actions in opposition to Gram-negative micro organism. Apparently, the addition of Fe(II) or Fe(III) ions may block the biofilm inhibition exercise of MaiA as a result of it’s an iron chelator. Nevertheless, not all iron chelators confirmed biofilm inhibition exercise, suggesting that MaiA prevents biofilm formation via a selected but presently undefined pathway. Moreover, MaiA acts as a synergist to boost colistin efficacy in opposition to Acinetobacter baumannii. Our outcomes point out that MaiA might doubtlessly function an efficient antibiofilm agent to stop Gram-negative biofilm formation in future medical functions.

Prevalence of Histidine Decarboxylase Genes of Gram-Constructive Micro organism in Surströmming as Revealed by qPCR

Histamine is a degradation product of the bacterial decarboxylation of the amino acid histidine; such exercise is set by histidine decarboxylase encoded by a gene cluster, carried by some Gram-positive micro organism, that features the hdcA gene. On this research, the presence of the hdcA gene in ready-to-eat surströmming samples collected from three producers based mostly in Sweden was straight assessed through qPCR evaluation for the very first time. Samples from producer A confirmed hdcA common gene abundance of 6.67 ± 0.13 Log cells/gene copies g-1; in samples from producer B the typical worth attested at 5.56 ± 0.06 Log cells/gene copies g-1, whereas for samples of producer C hdcA common gene abundance attested at 5.30 ± 0.08 Log cells/gene copies g-1.

ANOVA confirmed a considerably increased common hdcA gene copy quantity in samples from producer A, whereas no important variations had been seen between common values of hdcA gene copy numbers detected in samples from producer B and C. The hdcA gene copies detected within the current research may give an estimation of the load of potential histamine-producing micro organism in surströmming.

Antimicrobial Exercise of Lactococcus lactis subsp. lactis Remoted from a Stranded Cuvier’s Beaked Whale ( Ziphius cavirostris) in opposition to Gram-Constructive and -Adverse Micro organism

Within the current research, we remoted and characterised Lactococcus lactis (Llactis) subsp. lactis from a feminine Cuvier’s beaked whale (Ziphius cavirostris) stranded in Shizuoka, Japan. Solely 5 isolates (CBW1-5), grown on Lactobacilli de Man Rogosa Sharpe (MRS) agar plates ready utilizing 50% synthetic seawater, had been optimistic in Llactis species-specific primer PCR. Their 16S rRNA sequences had been extremely just like these of Llactis subsp. lactis JCM 5805T.

The Gram response, motility, fuel manufacturing from glucose, catalase manufacturing, and development circumstances had been in line with these of the kind pressure. Moreover, carbohydrate utilization of the strains was in line with beforehand reported marine organism-derived strains. The pH-neutralized cell-free tradition supernatant of pressure CBW2 inhibited the expansion of Bacillus subtilis subsp. subtilis ATCC 6051 and Vibrio alginolyticus ATCC 17749, whereas protease therapy eradicated or diminished its inhibitory exercise.

The pressure possesses a precursor of the nisin structural gene (nisA), which confirmed 100% homology with nisin Z, and nisin biosynthesis-related genes (nisBnisCnisTnisPnisFnisI, and nisRK), suggesting that the pressure produces a nisin-like substance. This research supplies elementary data on whale-derived Llactis subsp. lactis which can be helpful for decreasing the carriage of Bsubtilis subsp. subtilis and Valginolyticus.

Novel Extracellular Electron Switch Channels in a Gram-Constructive Thermophilic Bacterium

Biogenic transformation of Fe minerals, related to extracellular electron switch (EET), permits microorganisms to take advantage of high-potential refractory electron acceptors for vitality era. EET-capable thermophiles are dominated by hyperthermophilic archaea and Gram-positive micro organism. Data on their EET pathways is sparse. Right here, we describe EET channels within the thermophilic Gram-positive bacterium Carboxydothermus ferrireducens that drive exoelectrogenesis and speedy conversion of amorphous mineral ferrihydrite to giant magnetite crystals.

Maipomycin A, a Novel Natural Compound With Promising Anti-biofilm Activity Against Gram-Negative Pathogenic Bacteria

Microscopic research indicated biocontrolled formation of surprising formicary-like ultrastructure of the magnetite crystals and revealed energetic colonization of anodes in bioelectrochemical techniques (BESs) by C. ferrireducens. The inner construction of micron-scale biogenic magnetite crystals is reported for the primary time. Genome evaluation and expression profiling revealed three constitutive c-type multiheme cytochromes concerned in electron trade with ferrihydrite or an anode, sharing insignificant homology with beforehand described EET-related cytochromes thus representing novel determinants of EET. Our research determine these cytochromes as extracellular and reveal doubtlessly novel mechanisms of cell-to-mineral interactions in thermal environments.

Animal Fashions within the Analysis of the Effectiveness of Phage Remedy for Infections Attributable to Gram-Adverse Micro organism from the ESKAPE Group and the Reliability of Its Use in People

The authors emphasize how extraordinarily vital it’s to spotlight the position performed by animal fashions in an try to find out potential phage interactions with the organism into which it was launched in addition to to find out the protection and effectiveness of phage remedy in vivo taking into consideration the person circumstances of a given organism and its physiology.

Animal fashions by which phages are used make it potential, amongst different issues, to guage the efficient therapeutic dose and to decide on the potential route of phage administration relying on the kind of an infection developed. These outcomes can’t be utilized intimately to the human physique, however the data gained from animal experiments is invaluable and really useful.

Goat Anti-Human IgE (Epsilon-chain sp.)-FITC conjugate

10162 0.5 ml
EUR 225

Goat anti Human IgE (epsilon chain) (biotin)

43R-1130 1 mg
EUR 296
Description: Goat anti Human IgE (epsilon chain) secondary antibody (biotin)

Goat anti Human IgE (epsilon chain) (rhodamine)

43R-1132 1 mg
EUR 285
Description: Goat anti Human IgE (epsilon chain) secondary antibody (rhodamine)

Goat anti Human IgE (epsilon chain) (biotin)

43R-1135 1 mg
EUR 458
Description: Goat anti Human IgE (epsilon chain) secondary antibody (biotin)

Goat anti Human IgE (epsilon chain) (HRP)

43R-1137 1 mg
EUR 481
Description: Goat anti Human IgE (epsilon chain) secondary antibody (HRP)

Goat anti Human IgE (epsilon chain) (rhodamine)

43R-1138 1 mg
EUR 440
Description: Goat anti Human IgE (epsilon chain) secondary antibody (rhodamine)

Goat anti Human IgE (epsilon chain) (HRP)

43R-IG154hrp 1 mg
EUR 322
Description: Goat anti Human IgE (epsilon chain) secondary antibody (HRP)

Goat anti Human IgE (epsilon chain) (Alk Phos)

43R-1129 1 mg
EUR 380
Description: Goat anti Human IgE (epsilon chain) secondary antibody (Alk Phos)

Goat anti Human IgE (epsilon chain) (Alk Phos)

43R-1134 500 ug
EUR 380
Description: Goat anti Human IgE (epsilon chain) secondary antibody (Alk Phos)

Goat Anti-Human IgE (Epsilon-chain sp.)-HRP conjugate

10160 0.5 ml
EUR 213

Goat Anti-Human IgE (Epsilon-chain sp.)-AP conjugate

10161 0.5 ml
EUR 225

Goat Anti-Human IgE (Epsilon chain sp.) IgG-biotinylated

10163 0.5 ml
EUR 225

Goat Anti-Human IgE (Epsilon chain sp.) IgG, aff pure

10159 0.5 mg
EUR 202

Goat Anti-Mouse IgE (epsilon) Antibody

abx023395-1mg 1 mg
EUR 801
  • Shipped within 5-10 working days.

Goat anti Human IgE (FITC)

60-S2003GND1-AF 5 ml
EUR 241
Description: Goat anti Human IgE antibody (FITC)

Goat Anti-Mouse IgE-FITC

LF-SA8015F 1.0 mg
EUR 495
Description: Goat polyclonal to Mouse IgE

Goat anti-Human IgE Antibody (FITC)

abx401516-1mg 1 mg
EUR 495
  • Shipped within 1 week.

Goat Anti Human Ige Polyclonal Antibody,FITC

CPBT-65488GH 1 mg
EUR 710

Goat anti-Dog IgE Antibody (FITC)

abx401271-05mg 0.5 mg
EUR 565
  • Shipped within 1 week.

Goat, anti-human IgE

IM142 1 ml
EUR 359
  • Product category: Antibodies
Description: Goat, anti-human IgE by Cygnus Technologies is available in Europe via Gentaur.

Goat anti Human IgE

70R-IG015 1 mg
EUR 349
Description: Affinity purified Goat anti Human IgE antibody

Goat anti Human IgE

40-IG10 1 ml
EUR 133
Description: Goat anti Human IgE antibody

Goat anti Human IgE

20-S2001G000-S4 10 ml
EUR 127
Description: Goat anti Human IgE antibody

Goat anti Human IgE

20-S2001G000-V0 10 ml
EUR 133
Description: Goat anti Human IgE antibody

Goat anti Human IgE

20-S2001G000-VO 10 ml
EUR 133
Description: Goat anti Human IgE antibody

Goat anti Human IgE

20-S2003GND1-D0 10 ml
EUR 192
Description: Goat anti Human IgE antibody

Goat Anti-Human albumin IgG-FITC Conjugate

ALBH13-FITC 100 ug
EUR 347

Goat Anti Dog Ige Polyclonal Antibody,FITC

DPBT-67042GD 0.5 mg
EUR 715

Goat anti Human Kappa Chain (FITC)

43C-CB9122 1 mg
EUR 361
Description: Goat anti Human Kappa Chain secondary antibody (FITC)

Goat anti Human kappa chain (FITC)

43R-1259 500 ug
EUR 350
Description: Goat anti Human kappa chain secondary antibody (FITC)

Goat anti Human kappa chain (FITC)

43R-1265 500 ug
EUR 402
Description: Goat anti Human kappa chain secondary antibody (FITC)

Goat anti Human Lambda Chain (FITC)

60C-CB9129 1 mg
EUR 361
Description: Goat anti Human Lambda Chain secondary antibody (FITC)

Rat anti-Mouse IgE Heavy Chain Antibody (FITC)

abx401361-05mg 0.5 mg
EUR 718
  • Shipped within 1 week.

Goat Anti-Human IgE Antibody

abx023501-1mg 1 mg
EUR 599
  • Shipped within 5-10 working days.

Goat Anti-Human IgE Antibody

abx023502-10ml 10 ml
EUR 411
  • Shipped within 5-10 working days.

Goat anti-Human IgE Antibody

abx401259-1ml 1 ml
EUR 370
  • Shipped within 1 week.

Goat anti Canine IgE

70-IG84 1 mg
EUR 587
Description: Affinity purified Goat anti Canine IgE antibody

Anti-Hu IgE FITC

1F-324-C025 0.025 mg
EUR 108

Anti-Hu IgE FITC

1F-324-C100 0.1 mg
EUR 177

Anti-Hu IgE FITC

1F-326-C025 0.025 mg
EUR 108

Anti-Hu IgE FITC

1F-326-C100 0.1 mg
EUR 177

Goat Anti-Mouse albumin IgG-FITC Conjugate

ALB11-FITC 100 ug
EUR 347

Goat Anti-Dog IgE-FITC conjugate, aff pure

30393 0.5 ml
EUR 408

Rat Anti Mouse Ige Heavy Chain Monoclonal Antibody,FITC

CABT-54631RM 0.5 mg
EUR 741

Goat anti Human IgA (alpha chain) (FITC)

43C-CB9108 1 mg
EUR 186
Description: Goat anti Human IgA (alpha chain) secondary antibody (FITC)

Goat anti Human IgA (alpha chain) (FITC)

43C-CJ0102 500 ug
EUR 263
Description: Goat anti Human IgA (alpha chain) secondary antibody (FITC)

Goat anti Human IgA (alpha chain) (FITC)

43R-1121 1 mg
EUR 224
Description: Goat anti Human IgA (alpha chain) secondary antibody (FITC)

Goat anti Human IgM (mu chain) (FITC)

43R-1223 1 mg
EUR 138
Description: Goat anti Human IgM (mu chain) secondary antibody (FITC)

Goat anti Human IgM (mu chain) (FITC)

43R-1229 1 mg
EUR 137
Description: Goat anti Human IgM (mu chain) secondary antibody (FITC)

Goat anti Human IgM (mu chain) (FITC)

43R-1235 500 ug
EUR 263
Description: Goat anti Human IgM (mu chain) secondary antibody  (FITC)

Goat anti Human IgM (mu chain) (FITC)

43R-1241 500 ug
EUR 285
Description: Goat anti Human IgM (mu chain) secondary antibody  (FITC)

Goat Anti-Mouse IgG (Fc) IgG-FITC Conjugate

40151-FITC 1 ml
EUR 225

Goat Anti-Human Respiratory Syncytial Virus (RSV) IgG-FITC conjugate

RSV12-FITC 0.5 ml
EUR 469

Goat Anti-Human IgE (Fc) Antibody

abx023499-1mg 1 mg
EUR 356
  • Shipped within 5-10 working days.

Goat anti-Human IgE Antibody (AP)

abx401514-05mg 0.5 mg
EUR 495
  • Shipped within 1 week.

Goat anti-Human IgE Antibody (Biotin)

abx401515-1mg 1 mg
EUR 495
  • Shipped within 1 week.

Goat anti-Human IgE Antibody (HRP)

abx401517-1mg 1 mg
EUR 578
  • Shipped within 1 week.

Goat Anti Human Ige Polyclonal Antibody

CPBT-65485GH 1 ml
EUR 429

Goat Anti Human Ige Polyclonal Antibody

CPBT-65490GH 1 mg
EUR 663

Goat anti-Mouse IgE Antibody

abx401426-05mg 0.5 mg
EUR 704
  • Shipped within 1 week.

Goat anti-Mouse IgE Antibody

abx401524-1mg 1 mg
EUR 551
  • Shipped within 1 week.

Goat anti-Dog IgE Antibody

abx401270-05mg 0.5 mg
EUR 662
  • Shipped within 1 week.

Goat anti Canine IgE (HRP)

43R-IG011hrp 1 mg
EUR 739
Description: Goat anti Canine IgE secondary antibody (HRP)

Goat Anti-Mouse IgE-AP

LF-SA8015AP 1.0 ml
EUR 354
Description: Goat polyclonal to Mouse IgE

Goat Anti-Mouse IgE-BIOT

LF-SA8015B 1.0 mg
EUR 464
Description: Goat polyclonal to Mouse IgE

Goat Anti-Mouse IgE-HRP

LF-SA8015H 1.0 ml
EUR 334
Description: Goat polyclonal to Mouse IgE

Goat Anti-Mouse IgE-PE

LF-SA8015P 0.5 mg
EUR 644
Description: Goat polyclonal to Mouse IgE

Goat Anti-Mouse IgE-UNLB

LF-SA8015U 1.0 mg
EUR 429
Description: Goat polyclonal to Mouse IgE

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Rabbit Anti-Monkey IgE (epsilon chain sp) IgG, aff pure (Rhesus/cyano/baboon)

70051 100 ul
EUR 408

Rabbit Anti-Monkey (Rhesus/Cyno/Baboon) IgE (epsilon chain sp) IgG-HRP Conjugate

70051-HP 100 ul
EUR 469

Goat Anti-human C-Reactive Protein (CRP)-FITC Conjugate, aff pure

CRP11-FITC 100 ug
EUR 408

Goat Anti-Adenovirus type 2, hexon IgG-FITC conjugate

ADV11-FITC 0.5 ml
EUR 408

Goat Anti-Poliomyelitis Virus 1-3 IgG-FITC Conjugate

POLV13-FITC 0.5 ml
EUR 408

Goat Anti-Rubella virus (HPV77 strain) IgG-FITC conjugate

RUBL11-FITC 1 ml
EUR 347

FITC*Polyclonal Goat Anti-Human IgG(γ chain)

C030634-10ml 10ml
EUR 1368

FITC*Polyclonal Goat Anti-Human IgG(γ chain)

C030634-1ml 1ml
EUR 269

FITC*Polyclonal Goat Anti-Human IgM(u chain)

C030638-10ml 10ml
EUR 1368

FITC*Polyclonal Goat Anti-Human IgM(u chain)

C030638-1ml 1ml
EUR 269

Goat Anti-Human IgG gamma chain Antibody (FITC)

20-abx134829
  • EUR 258.00
  • EUR 356.00
  • 100 ul
  • 500 ul
  • Shipped within 5-10 working days.

Goat Anti-Human IgM mu chain Antibody (FITC)

20-abx134830
  • EUR 258.00
  • EUR 356.00
  • 100 ul
  • 500 ul
  • Shipped within 5-10 working days.

Goat anti Human Kappa Chain (Fab'2) (FITC)

43C-CJ0130 1 mg
EUR 385
Description: Goat anti Human Kappa Chain secondary antibody (Fab'2) (FITC)

Goat anti Human Lambda Chain (Fab'2) (FITC)

43C-CJ0135 1 mg
EUR 395
Description: Goat anti Human Lambda Chain secondary antibody (Fab'2) (FITC)

Goat Anti-Human IgG (gamma chain) antibody (FITC)

STJ99475-100l 100 µl
EUR 77
Description: FITC conjugated Goat polyclonal to Human IgG (gamma chain)

Goat Anti-Human IgG (gamma chain) antibody (FITC)

STJ99475-1mL 1 mL
EUR 195
Description: FITC conjugated Goat polyclonal to Human IgG (gamma chain)

Goat Anti-Human IgM (u chain) antibody (FITC)

STJ99478-100l 100 µl
EUR 89
Description: FITC conjugated Goat polyclonal to Human IgM (u chain)

Goat Anti-Human IgM (u chain) antibody (FITC)

STJ99478-1mL 1 mL
EUR 278
Description: FITC conjugated Goat polyclonal to Human IgM (u chain)

Anti-Rabbit Anti-Goat Heavy Chain Antibody FITC

STJ501162 100 µg
EUR 586

Anti-Rabbit Anti-Goat Light Chain Antibody FITC

STJ501177 100 µg
EUR 586

Goat anti Mouse IgM (mu chain) (FITC)

43R-1364 2 mg
EUR 296
Description: Goat anti Mouse IgM (mu chain) secondary antibody (FITC)

Goat anti Mouse IgM (mu chain) (FITC)

43R-1370 2 mg
EUR 322
Description: Goat anti Mouse IgM (mu chain) secondary antibody (FITC)

Human Immunoglobulin E (IgE) ELISA Kit

DLR-IgE-Hu-48T 48T
EUR 479
  • Should the Human Immunoglobulin E (IgE) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Immunoglobulin E (IgE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Immunoglobulin E (IgE) ELISA Kit

DLR-IgE-Hu-96T 96T
EUR 621
  • Should the Human Immunoglobulin E (IgE) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Immunoglobulin E (IgE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Immunoglobulin E (IgE) ELISA Kit

RDR-IgE-Hu-48Tests 48 Tests
EUR 500

Human Immunoglobulin E (IgE) ELISA Kit

RDR-IgE-Hu-96Tests 96 Tests
EUR 692

Goat Anti-Poly-His (6xhis) (His-tag) IgG-FITC conjugate

HISP16-FITC 100 ul
EUR 347

Goat Anti-Glutathione Transferase (GST, S. japonicum) IgG-FITC Conjugate

GST13-FITC 100 ul
EUR 347

Goat Anti-Hemeagglutinin fusion epitope (HA-tag) IgG-FITC conjugate

HA12-FITC 100 ug
EUR 347

Goat Anti-Human IgE (Fc) Antibody (HRP)

abx023500-1mg 1 mg
EUR 495
  • Shipped within 5-10 working days.

Goat Anti Human Ige Polyclonal Antibody,Biotin

CPBT-65487GH 1 mg
EUR 710

Goat Anti Human Ige Polyclonal Antibody,HRP

CPBT-65489GH 1 mg
EUR 819

Rabbit Anti-Monkey IgE (epsilon chain sp) IgG-Biotin, aff pure (Rhesus/cyano/baboon)

70051-BTN 100 ul
EUR 469

Goat Anti-OVA-IgE ELISA kit

E06O0333-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Goat Anti-OVA-IgE in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Anti-OVA-IgE ELISA kit

E06O0333-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Anti-OVA-IgE in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Anti-OVA-IgE ELISA kit

E06O0333-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Anti-OVA-IgE in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti-Mouse IgE Antibody (AP)

abx401427-05mg 0.5 mg
EUR 732
  • Shipped within 1 week.

Goat anti-Mouse IgE Antibody (HRP)

abx401428-05mg 0.5 mg
EUR 815
  • Shipped within 1 week.

Goat anti-Dog IgE Antibody (HRP)

abx401272-05mg 0.5 mg
EUR 606
  • Shipped within 1 week.

Goat Anti-Horse IgE-HRP conjugate

80871 0.5 ml
EUR 347

We wish to spotlight how helpful animal fashions could also be for the potential effectiveness analysis of phage remedy within the case of infections brought on by gram-negative micro organism from the ESKAPE (Enterococcus faeciumStaphylococcus aureusKlebsiella pneumoniaeAcinetobacter baumanniiPseudomonas aeruginosaEnterobacter species) group of pathogens. On this overview, we focus particularly on the info from the previous few years.

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